Common hematological parameters reference intervals for apparently healthy pregnant and non-pregnant women of South Wollo Zone, Amhara Regional State, Northeast Ethiopia

• DOI: 10.1371/journal.pone.0270685
• Publication Date: 2022-07-15T17:30:24Z
• Authors: Mesfin Fiseha; Miftah Mohammed; Endris Ebrahim; Wondmagegn Demsiss; Mohammed Tarekegn; Amanuel Angelo; Mikias Negash; Zemenu Tamir; Mihret Tilahun; Aster Tsegaye
• Keywords: Science; Q; R; Blood Cell Count; 3. Good health; Leukocyte Count; 03 medical and health sciences; Cross-Sectional Studies; 0302 clinical medicine; Pregnancy; Reference Values; Medicine; Humans; Female; Ethiopia; Research Article

Background Hematological reference intervals (RIs) are affected by inherent variables like age, sex, genetic background, environment, diet and certain circumstances such as pregnancy signifying the need for population specific values. This study was designed to establish RIs for common hematological parameters of apparently healthy pregnant and non-pregnant women from Northeast Ethiopia.

Method This community based cross-sectional study recruited 600 pregnant and non-pregnant women in South Wollo Zone, Northeast Ethiopia from June to August 2019. Complete blood count was performed for eligible participants using Mindary BC-3000 plus hematology analyzer. The mean, median, and 2.5th and 97.5th percentile reference limits with 90% CI were determined using SPSS version 23.

Result The established selected 2.5th–97.5th percentiles RIs for pregnant women were: WBC: 4.0–13.2x109/L; RBC: 3.45–4.67x1012/L; Hgb: 10.1–13.7g/dL; HCT: 33.5–46.5%; MCV: 85-104fL; MCH: 27.5–33.0pg; MCHC: 30.3–33.7g/dL and Platelet count: 132-373x109/L. The respective values for non-pregnant women were 3.6–10.3; 4.44–5.01; 12.4–14.3; 38.4–50.1; 86–102; 27.1–32.4, 30.4–34.1, 173–456. A statistically significant difference between pregnant and non-pregnant women was noted in all hematological parameters except MCHC. The mean and median value of WBC count, MCV, MPV and PDW increased, whereas mean values of HCT and Platelet count decreased as gestational period advances.

Conclusion The observed difference from other studies signify the necessity for using trimester specific RIs and separate RI for pregnant and non-pregnant women. Thus, we recommend the health facilities in the study area to utilize the currently established RIs for pregnant and non-pregnant women for better care.