β-tricalcium phosphate/gelatin composite scaffolds incorporated with gentamycin-loaded chitosan microspheres for infected bone defect treatment

• DOI: 10.1371/journal.pone.0277522
• Publication Date: 2022-12-08T18:28:17Z
• Authors: Yu Liu; Qingqing Zhao; Changsong Chen; Chunhu Wu; Yuhai Ma
• Keywords: Gelatin

The repair of infected bone defects remains a clinical challenge. Staphylococcus aureus is common pathogenic micro-organism associated with such infections. Gentamycin (GM) broad spectrum antibiotic that can kill S . in dose-dependent manner. However, the systemic administration antibiotics may lead to drug resistance and gut dysbiosis. In this work, we constructed β-tricalcium phosphate/gelatin composite scaffolds incorporated gentamycin-loaded chitosan microspheres (CMs(GM)-β-TCP/gelatin scaffolds), which helped optimize local GM release defect areas enhance regeneration. cumulative curves showed both reached sustained slow-release phase after initial rapid release, latter further stabilized rate. curve CMs(GM)-β-TCP/gelatin plateau 24 h, 41.86% during period. Moreover, combination β-TCP gelatin mimicked composition were able provide requisite mechanical strength (0.82 ± 0.05 MPa) first generation. inner structure scaffold was arranged shape interconnected pores, presented porosity level 16%. apertures uniform size, beneficial for cell proliferation material transportation. Macroscopic observation histological analysis fused tissues, new tissues formed without any infection. This be promising treating defects.