cGMP dynamics that underlies thermosensation in temperature-sensing neuron regulates thermotaxis behavior in C. elegans

• DOI: 10.1371/journal.pone.0278343
• Publication Date: 2022-12-06T18:35:30Z
• Authors: Ichiro Aoki; Makoto Shiota; Yuki Tsukada; Shunji Nakano; Ikue Mori
• Keywords: Neurons; 0303 health sciences; 03 medical and health sciences; Science; Q; R; Medicine; Animals; Caenorhabditis elegans; Research Article

Living organisms including bacteria, plants and animals sense ambient temperature so that they can avoid noxious or adapt to new environmental temperature. A nematode C. elegans innocuous temperature, navigate themselves towards memorize past cultivation (Tc) of their preference. For this thermotaxis, AFD thermosensory neuron is pivotal, which stereotypically responds warming by increasing intracellular Ca2+ level in a manner dependent on the remembered Tc. We aimed reveal how encodes information into neural activities. cGMP synthesis crucial for thermosensation thermotaxis behavior. Here we characterized dynamic change imaging expressing fluorescence resonance energy transfer (FRET)-based probe specifically found dynamically responded both cooling Moreover, mutant lack guanylyl cyclases (GCYs) phosphodiesterases (PDEs), synthesize hydrolyze cGMP, respectively, uncovered GCYs PDEs contribute dynamics