Genome-wide association mapping for component traits of drought tolerance in dry beans (Phaseolus vulgaris L.)

• DOI: 10.1371/journal.pone.0278500
• Publication Date: 2023-05-18T17:24:39Z
• Authors: Bruce Mutari; Julia Sibiya; Admire Shayanowako; Charity Chidzanga; Prince M. Matova; Edmore Gasura
• Keywords: Drought Tolerance; Genetic Association

Understanding the genetic basis of traits economic importance under drought stressed and well-watered conditions is important in enhancing gains dry beans ( Phaseolus vulgaris L.). This research aims to: (i) identify markers associated with agronomic physiological for tolerance (ii) drought-related putative candidate genes within mapped genomic regions. An andean middle-american diversity panel (AMDP) comprising 185 genotypes was screened field two successive seasons. Agronomic traits, viz ., days to 50% flowering (DFW), plant height (PH), maturity (DPM), grain yield (GYD), 100-seed weight (SW), leaf temperature (LT), chlorophyll content (LCC) stomatal conductance (SC) were phenotyped. Principal component association analysis conducted using filtered 9370 Diversity Arrays Technology sequencing (DArTseq) markers. The mean PH, GYD, SW, DPM, LCC SC reduced by 12.1, 29.6, 10.3, 12.6, 28.5 62.0%, respectively conditions. Population structure revealed sub-populations, which corresponded gene pools. Markers explained 0.08–0.10, 0.22–0.23, 0.29–0.32, 0.43–0.44, 0.65–0.66 0.69–0.70 total phenotypic variability R 2 ) SC, LT, SW DFW, For conditions, varied from 0.08 (LT) 0.70 (DPM). Overall, 68 significant (p < 10 −03 marker-trait associations (MTAs) 22 identified across Most had known biological functions related regulating response stress. findings provide new insights into architecture stress common bean. also potential SNPs that can be utilized discovery marker-assisted breeding after validation.