The bacterial profile and antibiotic susceptibility pattern in respiratory tract samples from art-experienced HIV-positive adults in Uganda

• DOI: 10.1371/journal.pone.0282936
• Publication Date: 2023-08-31T17:25:52Z
• Authors: Gloria Lubega; Andrew Abaasa; Willyfred Ochola; Bernard Kikaire; Joseph Lutaakome; Eugene Rugazira; Yunia Mayanja
• Keywords: Adult; Male; Staphylococcus aureus; Bacteria; Science; Q; R; Middle Aged; Amoxicillin-Potassium Clavulanate Combination; Haemophilus influenzae; 3. Good health; Anti-Bacterial Agents; Pseudomonas; HIV Seropositivity; Drug Resistance, Bacterial; Escherichia coli; Medicine; Humans; Moraxella; Female; Uganda; Research Article; Retrospective Studies

IntroductionMicrobial infections are a major cause of morbidity and mortality among people living with HIV (PLWH). Respiratory tract infections (RTIs) are responsible for approximately 70% of illnesses among PLWH. Drug resistant bacteria are highly prevalent among PLWH and this is a public health concern.MethodsThis is a retrospective analysis of data collected during the COSTOP trial between 2011 and 2013. Sputum collected on spot from participants presenting with a productive cough was examined using Gram, Ziehl-Neelsen stains and cultured on suitable bacteriological media. Antimicrobial sensitivity testing was done on isolated pathogens, by disc diffusion technique.ResultsWe included 687 participants with mean age 41.3 (SD 8.2) years of whom 76.4% were female. Two hundred one sputum samples grew bacteria;Moraxella species(27.4%),Streptococcus pneumoniae(25.4%),Haemophilus influenza(22.4%),Mycobacterium species(4.5%),Pseudomonas species(4.0%),Staphylococcus aureus(4.0%),Escherichia coli(1.0%),Klebsiella species(1.0%), other bacteria (10.4%). A higher monthly income greater than or equal to 30$ (aOR = 0.63, 95%CI: 0.40–0.99) and longer duration since HIV diagnosis (aOR = 1.06, 95%CI: 1.0–1.11) were found to be independently associated with a positive bacterial culture. Moraxellasp, H.influenzaand Pseudomonas had zero sensitivity towards cotrimoxazole. Sensitivity to erythromycin was low among Moraxellasp(28.6%), H.influenza(31.6%) and S.aureus(42.9%) and other bacteria (42.9%). Most isolates were sensitive to Amoxicillin + Clavulanic acid and ceftriaxone.ConclusionThere is a very low sensitivity of isolated bacteria to commonly prescribed antibiotics that are more available through the national supply chain, which is of public health concern. Urgent steps to tackle the high antimicrobial resistance among PLWH is required.