Mycobacteria modulate SUMOylation to suppresses protective responses in dendritic cells

• DOI: 10.1371/journal.pone.0283448
• Publication Date: 2023-09-29T17:23:06Z
• Authors: Vandana Anang; Aayushi Singh; Ankush Kumar Rana; Shakuntala Surend...; Upasana Bandyopad...; Chaitenya Verma; Attinder Chadha; Krishnamurthy Nat...
• Keywords: Mycobacterium Infections; Science; Q; R; Sumoylation; Dendritic Cells; Mycobacterium bovis; 3. Good health; Mice; Medicine; Animals; Cytokines; Female; Research Article

Post translational modifications (PTMs) are exploited by various pathogens in order to escape host immune responses. SUMOylation is one of the PTMs which involved regulation a variety cellular However, effects on pathogenic bacteria largely remain elusive. We, therefore, investigated role regulating defense responses dendritic cells (DCs) during mycobacterial infection. Dendritic Cells female BALB/c mice and THP-1 macrophages were used. Western blotting was performed measure expression level SUMO1, pSTAT1, pp38, pERK, Beclin-1, LC3, Bax Cytochrome C. For bacterial burden confocal microscopy CFU (Colony Forming Unit) Flow cytometry used for ROS co-stimulatory molecules measurement. Cytokine measured using ELISA. We show that stimulation Bone Marrow Derived (BMDCs) with antigen Rv3416 or live infection Mycobacterium bovis BCG increases proteins. Inhibition significantly decreased intracellular loads DCs. Additionally, inhibiting SUMOylation, induces protective increasing oxidative burst, pro-inflammatory cytokine surface T cell molecules, activation pSTAT1 Mitogen Activated Protein Kinases (MAPK) proteins- pp38 pERK. inhibition also increased apoptosis autophagy BMDCs. Intriguingly, mycobacteria many above molecules. Furthermore, DCs primed turn attenuated infected macrophages. These findings demonstrate pathway thwart