Immunoregulatory properties of erythroid nucleated cells induced from CD34+ progenitors from bone marrow

• DOI: 10.1371/journal.pone.0287793
• Publication Date: 2023-06-30T17:29:49Z
• Authors: Julia A. Shevchenko; Roman Yu Perik-Za...; Kirill V. Nazarov; Vera V. Denisova; Olga Yu. Perik-Za...; Yulia G. Philippova; Alaa Alsalloum; Sergey V. Sennikov
• Keywords: Transferrin receptor

CD 71+ erythroid nucleated cells have pronounced immunoregulatory properties in normal and pathological conditions. Many populations of with are considered candidates for cellular immunotherapy various pathologies. This study characterized the CD71+ derived from CD34-positive bone marrow under influence growth factors that stimulate differentiation into cells. CD34-negative were used to isolate nuclear The resulting assess phenotype, determine mRNA spectrum genes responsible main pathways processes immune response, obtain culture supernatants analysis factors. It was found CD34+ carry markers cells, but differ markedly natural differences presence CD45+ subpopulation, distribution terminal stages, transcriptional profile, secretion certain cytokines, immunosuppressive activity. induced closer extramedullary erythropoiesis foci than Thus, when cultivating clinical experimental studies, it is necessary take account their