QTL mapping of oleic acid content in modern VNIIMK sunflower (Helianthus annuus L.) lines by using GBS-based SNP map

• DOI: 10.1371/journal.pone.0288772
• Publication Date: 2023-10-04T17:40:14Z
• Authors: Rim Gubaev; Stepan Boldyrev; Elena Martynova; Alina Chernova; Tatyana Kovalenko; Yuliya Chebanova; Tatyana Peretyagina; Svetlana Goryunova; Denis Goryunov; Zhanna Mukhina; Cecile Ben; Laurent Gentzbittel; Philipp Khaitovich; Yakov Demurin
• Keywords: Helianthus annuus

Oleic acid is a monounsaturated fatty increasing oil oxidative stability. High content of oleic thus valuable trait in oilseed crops. Sunflower ( Helianthus annuus L.) normally accumulates linoleic as major acid, but mutant expressing high phenotype form was previously obtained by chemical mutagenesis and mapped on the sunflower genome. Several studies suggest presence additional genes involved control with their expression possibly depending genetic background. To test this hypothesis, we performed QTL mapping within two independent F 2 crosses involving lines contrasting from Pustovoit All-Russia Research Institute Oil Crops (VNIIMK) collection. We applied genotyping-by-sequencing (GBS) to construct single nucleotide polymorphism-based maps using quantitative qualitative encoding for content. Our results support finding that assessed controlled one effect locus. However, different dominant/recessive effects locus were reported both crosses. Additionally, possible translocation between chromosome 7 14 cross. defined set polymorphism markers each cross which could be used marker-assisted selection.