Antioxidant and antiproliferative effect of a glycosaminoglycan extract from Rapana venosa marine snail

• DOI: 10.1371/journal.pone.0297803
• Publication Date: 2024-02-15T18:42:20Z
• Authors: Alexandra Gaspar-...; Laura M. Stefan; Elena Mihai; Catalina Sanda; Vasile S. Manoiu; Daniela Berger; Oana Craciunescu
• Keywords: Agarose gel electrophoresis

Marine glycosaminoglycans (GAG) isolated from different invertebrates, such as molluscs, starfish or jellyfish, have been described unique molecules with important pharmacological applications. Scarce information is available on GAG extract Rapana venosa marine snail. The aim of this study was to isolate a R . snail and investigate its physicochemical, antioxidant antiproliferative properties for further biomedical use. morphology, chemical elemental composition the were established well sulfate content N- O-sulfation ratio. Fourier transform infrared (FTIR) spectra indicated that presented similar structural characteristics bovine heparan chondroitin sulfate. pattern migration in agarose gel electrophoresis specific digestion chondroitinase ABC heparinase III presence mixture sulfate-type GAG, main component, GAG. Free radical scavenging ferric ion reducing assays showed had high activity, which slightly decreased after enzymatic treatment. In vitro MTT Live/Dead ability inhibit cell proliferation human Hep-2 cultures, at cytocompatible concentrations normal NCTC clone L929 fibroblasts. This capacity digestion, accordance activity products. Tumoral also inhibited by Overall, exhibited activities, suggesting potential use novel bioactive compound