Sound waves alter the viability of tobacco cells via changes in cytosolic calcium, membrane integrity, and cell wall composition

• DOI: 10.1371/journal.pone.0299055
• Publication Date: 2024-03-11T17:30:48Z
• Authors: Mahsa Sardari; Faezeh Ghanati; Hamid Mobasheri; Abazar Hajnorouzi
• Keywords: Phenylalanine ammonia-lyase

The effect of sound waves (SWs) on plant cells can be considered as important other mechanical stimuli like touch, wind, rain, and gravity, causing certain responses associated with the downstream signaling pathways whole plant. objective present study was to elucidate response suspension-cultured tobacco ( Nicotiana tabacum L. cv Burley 21) SW at different intensities. sinusoidal (1,000 Hz) produced through a signal generator, amplified, beamed one layer floating inside soundproof chamber intensities 60, 75, 90 dB plate level for 15, 30, 45, 60 min. Calibration applied intensities, accuracy, uniformity performed by meter, were treated. monitored quantitation cytosolic calcium, redox status, membrane integrity, wall components, activity modifying enzymes. Cytosolic calcium ions increased function intensity maximum dB. Exposure also accompanied significant increase H 2 O lipid peroxidation rate but reduction total antioxidant radical scavenging capacities. rigidity in these attributed an wall-bound phenolic acids lignin activities phenylalanine ammonia-lyase covalently bound peroxidase. In comparison, 60- 75 dB, capacity increased, stiffening enzymes reduced, cell viability showed no changes. outcome current reveals that impact is started calcium. However, upon signaling, events, including alteration status enzymes, determined extent effects cells.