Data retrieval from archival renal biopsies using nonlinear microscopy

• DOI: 10.1371/journal.pone.0299506
• Publication Date: 2024-03-15T17:46:47Z
• Authors: Lucas C. Cahill; Tadayuki Yoshitake; Milan Rosen; Timothy D. Weber; James G. Fujimoto; Seymour Rosen
• Keywords: Microtome; Clearance

Thorough examination of renal biopsies may improve understanding disease. Imaging with fluorescence nonlinear microscopy (NLM) and optical clearing enables three-dimensional (3D) visualization pathology without microtome sectioning. Archival paraffin blocks from 12 patients were deparaffinized stained Hoechst Eosin for fluorescent nuclear cytoplasmic/stromal contrast, then optically cleared using benzyl alcohol benzoate (BABB). NLM images entire biopsy fragments (thickness range 88-660 μm) acquired signals mapped to an H&E color scale. Cysts, glomeruli, exudative lesions, Kimmelstiel-Wilson nodules segmented in 3D their volumes, diameters, percent composition could be obtained. The glomerular count on volumes was high indicating that archival a vast tissue resource enable larger-scale retrospective studies. Rapid imaging more thorough is promising technique analysis blocks.