Characterizing medaka visual features using a high-throughput optomotor response assay

• DOI: 10.1371/journal.pone.0302092
• Publication Date: 2024-06-28T18:31:17Z
• Authors: Risa Suzuki; Jia Zheng Woo; Thomas Thumberger; Gero Hofmann; Joachim Wittbrodt; Tinatini Tavhelid...
• Keywords: Oryzias

Medaka fish (Oryzias latipes) is a powerful model to study genetics underlying the developmental and functional traits of vertebrate visual system. We established simple high-throughput optomotor response (OMR) assay utilizing medaka larvae functions including acuity contrast sensitivity. Our presents multiple adjustable stripes in motion individual linear arena. For that OMR employs tablet display Fish Stripes software adjust speed, width, color, stripes. results demonstrated responses were robustly induced by black white presented from below linear-pool-arena. detected robust strain specific differences when comparing long inbred strains. observed an interesting training effect upon initial exposure thick stripes, which allowed them better respond narrower The setup protocol here provide efficient tool for quantitative phenotype mapping, addressing acuity, trainability cortical neurons, color sensitivity, locomotor response, retinal regeneration others. open-source provides crucial prerequisite ultimately genetic basis those processes.