Global biochemical profiling of fast-growing Antarctic bacteria isolated from meltwater ponds by high-throughput FTIR spectroscopy

• DOI: 10.1371/journal.pone.0303298
• Publication Date: 2024-06-17T18:01:31Z
• Authors: Volha Akulava; Valeria Tafintseva; Uladzislau Blazhko; Achim Kohler; Uladzislau Miamin; Leonid Valentovich; Volha Shapaval
• Keywords: 0301 basic medicine; Principal Component Analysis; 03 medical and health sciences; Bacteria; Spectroscopy, Fourier Transform Infrared; Temperature; Antarctic Regions; Ponds; Water Microbiology; Phylogeny; Research Article

Fourier transform infrared (FTIR) spectroscopy is a biophysical technique used for non-destructive biochemical profiling of biological samples. It can provide comprehensive information about the total cellular profile microbial cells. In this study, FTIR was to perform characterization twenty-nine bacterial strains isolated from Antarctic meltwater ponds. The bacteria were grown on two forms brain heart infusion (BHI) medium: agar at six different temperatures (4, 10, 18, 25, 30, and 37°C) broth 18°C. Multivariate data analysis approaches such as principal component (PCA) correlation study difference in profiles induced by cultivation conditions. observed results indicated strong between spectra phylogenetic relationships among studied bacteria. most accurate taxonomy-aligned clustering achieved with cultivated agar. Cultivation BHI medium provided biochemically biomass. impact temperature species-specific, however, similarly all bacteria, lipid spectral region least affected while polysaccharide temperatures. biggest temperature-triggered changes cell chemistry detected wide tolerance Pseudomonas lundensis Acinetobacter lwoffii BIM B-1558.