The protective effect of DMI on hippocampus EEG, behavioral and biochemical parameters in hypoxia-induced seizure on neonatal period

• DOI: 10.1371/journal.pone.0309240
• Publication Date: 2024-11-04T18:27:10Z
• Authors: Shadi Nazarizadeh; Zohreh Ghotbeddin; Samireh Ghafouri; Alireza Sarkaki
• Keywords: Hypoxia; Elevated plus maze; Neonatal seizure; Motor coordination

Hypoxia-Induced Neonatal Seizure (HINS) is a prevalent type of seizure in infants caused by hypoxic conditions, which can lead to an increased risk epilepsy, learning disabilities, and cognitive impairments later life. This study focuses on examining the effects dimethyl itaconate (DMI) cognition, motor coordination, anxiety-like behavior male rats that have experienced HINS. 42 Wistar newborn (PND10) were randomly divided into six groups (n = 7). 1) Control (Vehicle only); received DMI solvent (0.1ml) without applying hypoxia. 2–3) DMI; receiving (20 50 mg/kg; i.p). 4) HINS; they placed hypoxia chamber with 7% oxygen 93% nitrogen concentration for 15 minutes. 5–6) DMI+HINS; i.p) 24h before Behavioral tests including; Novel object recognition test, Rotarod, Parallel bar, Open field elevated plus maze (EPM); started at age 45 after birth. After behavioral tests, hippocampal CA1 region local EEG was recorded all groups. Then brain hippocampus tissue isolated amount MDA, SOD, NO, Thiol measured ELISA method. Data showed administration improved symptoms, behaviors, cognition HINS (p<0.05). power group decreased significantly compared other experimental Biochemical observations reduced oxidative stress inflammation Increased be effective occurrence disorders observed rats. While these reducing inflammation.