The Arabidopsis Resistance-Like Gene SNC1 Is Activated by Mutations in SRFR1 and Contributes to Resistance to the Bacterial Effector AvrRps4

• DOI: 10.1371/journal.ppat.1001172
• Publication Date: 2010-11-04T15:49:16Z
• Authors: Sang Hee Kim; Fei Gao; Saikat Bhattacharjee; Joseph A. Adiasor; Ji Chul Nam; Walter Gassmann
• Keywords: Nicotiana; 0303 health sciences; Sequence Homology, Amino Acid; QH301-705.5; Arabidopsis Proteins; Immunoblotting; Molecular Sequence Data; Arabidopsis; RC581-607; Plants, Genetically Modified; Immunity, Innate; 3. Good health; Plant Leaves; 03 medical and health sciences; Phenotype; Bacterial Proteins; Gene Expression Regulation, Plant; Mutation; Amino Acid Sequence; Immunologic diseases. Allergy; Biology (General); Cloning, Molecular; Research Article; Plant Diseases

The SUPPRESSOR OF rps4-RLD1 (SRFR1) gene was identified based on enhanced AvrRps4-triggered resistance in the naturally susceptible Arabidopsis accession RLD. No other phenotypic effects were recorded, and extent of SRFR1 involvement regulating effector-triggered immunity unknown. Here we show that mutations Columbia-0 (Col-0) lead to severe stunting constitutive expression defense PR1. These phenotypes temperature-dependent. A cross between srfr1-1 (RLD background) srfr1-4 showed caused by a recessive locus Col-0. Mapping targeted crosses Col-0-specific SNC1 as causes stunting. proposed function transcriptional repressor, is indeed overexpressed srfr1-4. Interestingly, co-regulated genes cluster are also upregulated snc1-11 double mutant, indicating overexpression not secondary effect activation. In addition, Col-0 RPS4 mutant full susceptibility bacteria expressing avrRps4 at 24°C but 22°C, while RLD rps4-2 increased, full, additional cross-talk pathways may exist. Intriguingly, when transiently expressed Nicotiana benthamiana, SRFR1, common protein complex cytoplasmic microsomal compartment. Our results highlight convergence point least subset TIR-NBS-LRR protein-mediated Arabidopsis. Based evident from our results, they suggest reports need consider possible SNC1.