Structure-Function Analysis of Barley NLR Immune Receptor MLA10 Reveals Its Cell Compartment Specific Activity in Cell Death and Disease Resistance

• DOI: 10.1371/journal.ppat.1002752
• Publication Date: 2012-06-07T21:23:35Z
• Authors: Shiwei Bai; Jie Liu; Cheng Chang; Ling Zhang; Takaki Maekawa; Qiuyun Wang; Wenkai Xiao; Yule Liu; Jijie Chai; Frank L. W. Takken; Paul Schulze-Lefert; Qian-Hua Shen
• Keywords: Nuclear export signal; Immune receptor

Plant intracellular immune receptors comprise a large number of multi-domain proteins resembling animal NOD-like (NLRs). NLRs typically recognize isolate-specific pathogen-derived effectors, encoded by avirulence (AVR) genes, and trigger defense responses often associated with localized host cell death. The barley MLA gene is polymorphic in nature encodes the coiled-coil (CC)-NB-LRR type that each detects cognate effector powdery mildew fungus. We report systematic analyses MLA10 activity disease resistance death signaling Nicotiana benthamiana. CC domain-triggered regulated highly conserved motifs NB-ARC domains C-terminal LRR receptor. Enforced subcellular localization, tagging nuclear localization sequence (NLS) or export (NES), shows suppressed nucleus but enhanced cytoplasm. By contrast, sufficient to mediate against retention cytoplasm was achieved through attachment glucocorticoid receptor hormone-binding domain (GR), which we reinforced role cytoplasmic signaling. Together our data showing an essential resistance, this suggests bifurcation MLA10-triggered compartment-dependent manner.