The Interactomes of Influenza Virus NS1 and NS2 Proteins Identify New Host Factors and Provide Insights for ADAR1 Playing a Supportive Role in Virus Replication

• DOI: 10.1371/journal.ppat.1003440
• Publication Date: 2013-07-04T22:31:18Z
• Authors: Benoît de Chassey; Anne Aublin-Gex; Alessia Ruggieri; Laurène Meyniel-S...; Fabrine Pradezynski; Nathalie Davoust; Thibault Chantier; Lionel Tafforeau; Philippe-Emmanuel...; Claire Ciancia; Laure Perrin-Cocon; Ralf Bartenschlager; Patrice André; Vincent Lotteau
• Keywords: Host factors; VP40; Viral structural protein; Heterogeneous nuclear ribonucleoprotein

Influenza A NS1 and NS2 proteins are encoded by the RNA segment 8 of viral genome. is a multifunctional protein virulence factor while involved in nuclear export ribonucleoprotein complexes. yeast two-hybrid screening strategy was used to identify host factors supporting functions. More than 560 interactions between 79 cellular from 9 different influenza virus strains have been identified. These interacting potentially each step infectious process their contribution replication tested interference. Validation relevance these cell for cycle revealed that 7 and/or NS2-interacting positively or negatively controlled replication. One main targeted all double-stranded binding domain family. In particular, adenosine deaminase acting on 1 (ADAR1) appeared as pro-viral whose expression necessary optimal synthesis Surprisingly, ADAR1 also dengue directly interacted with NS3 protein. editing activity enhanced both viruses through proteins, suggesting similar virus-host co-evolution.