Quantitative Proteomics Uncovers Novel Factors Involved in Developmental Differentiation of Trypanosoma brucei

• DOI: 10.1371/journal.ppat.1005439
• Publication Date: 2016-02-24T22:52:59Z
• Authors: Mario Dejung; Ines Subota; Ferdinand Bucerius; Gülcin Dindar; Anja Freiwald; Markus Engstler; Michael Boshart; Falk Butter; Christian J. Janzen
• Keywords: PRC2; Proteome; Developmental Biology

Developmental differentiation is a universal biological process that allows cells to adapt different environments perform specific functions. African trypanosomes progress through tightly regulated life cycle in order survive host when they shuttle between an insect vector and vertebrate host. Transcriptomics has been useful gain insight into RNA changes during stage transitions; however, levels are only moderate proxy for protein abundance trypanosomes. We quantified 4270 groups from the mammalian-infective form provide classification their expression profiles development. Our label-free quantitative proteomics study revealed previously unknown components of machinery involved essential processes such as signaling, posttranslational modifications, trafficking nuclear transport. Furthermore, guided by our proteomic survey, we identified cause observed impairment histone methyltransferase DOT1B knock-out strain it required accurate karyokinesis first cell division differentiation. This epigenetic regulator likely chromatin restructuring developmental differentiation, which might also be important higher eukaryotic cells. proteome dataset will serve resource detailed investigations shed more light on molecular mechanisms this other eukaryotes.