MUC1 is a receptor for the Salmonella SiiE adhesin that enables apical invasion into enterocytes

• DOI: 10.1371/journal.ppat.1007566
• Publication Date: 2019-02-04T18:45:48Z
• Authors: Xinyue Li; Nancy M. C. Bleum...; Yvette M. C. A. L...; Richard W. Wubbolts; Jos P. M. van Putten; Karin Strijbis
• Keywords: MUC1; Apical membrane

The cellular invasion machinery of the enteric pathogen Salmonella consists a type III secretion system (T3SS) with injectable virulence factors that induce uptake by macropinocytosis. at apical surface intestinal epithelial cells is inefficient, presumably because glycosylated barrier formed transmembrane mucins prevents T3SS contact host cells. We observed capable express mucin MUC1. Knockout MUC1 in HT29-MTX or removal sialic acids neuraminidase treatment reduced but did not affect lateral hampered defensive barrier. A deletion strain lacking SiiE giant adhesin was unable to invade through SiiE-positive closely associated layer surface, invaded were negative for adhesin. Our findings uncover required SiiE-mediated entry enterocytes via route.