Pathogenicity island excision during an infection by Salmonella enterica serovar Enteritidis is required for crossing the intestinal epithelial barrier in mice to cause systemic infection

• DOI: 10.1371/journal.ppat.1008152
• Publication Date: 2019-12-04T13:38:09Z
• Authors: Catalina Pardo-Roa; Geraldyne A. Salazar; Loreani P. Noguera; Francisco J. Sala...; Omar P. Vallejos; Isidora D. Suazo; Bárbara M. Schultz; Irenice Coronado-...; Alexis M. Kalergis; Susan M. Bueno
• Keywords: Salmonella enteritidis; Pathogenicity island; Salmonella enterica; Mesenteric lymph nodes; Salmonella infection

Pathogenicity island excision is a phenomenon that occurs in several Salmonella enterica serovars and other members of the family Enterobacteriaceae. ROD21 an excisable pathogenicity found chromosome S. Enteritidis, Dublin Typhi among others, which contain genes encoding virulence-associated proteins. Excision may play role ability Enteritidis to cause systemic infection mice. Our previous studies have shown strains unable excise display reduced colonize liver spleen. In this work, we determined kinetics vivo C57BL/6 mice its effect on virulence. We quantified bacterial burden frequency different portions digestive tract internal organs throughout infection. observed was significantly increased population colonizing mesenteric lymph nodes at early stages infective cycle, before 48 hours post-infection. contrast, remained very low spleen these stages. Interestingly, drastically after h post infection, when intestinal re-infection mortality begun. Moreover, inability had negative capacity translocate from intestine deeper organs, correlates with abnormal transcription invA strain ROD21. These results suggest genetic mechanism required by produce successful invasion epithelium, step generate