PET/CT targeted tissue sampling reveals virus specific dIgA can alter the distribution and localization of HIV after rectal exposure

• DOI: 10.1371/journal.ppat.1009632
• Publication Date: 2021-06-01T20:10:17Z
• Authors: Roslyn A. Taylor; Sixia Xiao; Ann M. Carias; Michael D. McRaven; Divya N. Thakkar; Mariluz Araínga; Edward J. Allen; Kenneth A. Rogers; Sidath C. Kumarap...; Siqi Gong; Angela J. Fought; Meegan R. Anderson; Yanique Thomas; Jeffrey R. Schneider; Beth Goins; Peter Fox; Francois J. Villi...; Ruth M. Ruprecht; Thomas J. Hope
• Keywords: Mesenteric lymph nodes

Human immunodeficiency virus (HIV) vaccines have not been successful in clinical trials. Dimeric IgA (dIgA) the form of secretory is most abundant antibody class mucosal tissues, making dIgA a prime candidate for potential HIV vaccines. We coupled Positron Emission Tomography (PET) imaging and fluorescent microscopy 64Cu-labeled, photoactivatable-GFP (PA-GFP-BaL) fluorescently labeled to determine how antibodies influence interaction with barriers viral penetration colorectal tissue. Our results show that virions rapidly disseminate throughout colon two hours after exposure. The presence resulted an increase depth transverse colon. Moreover, were found mesenteric lymph nodes exposure, led nodes. Taken together, these technologies enable vivo situ visualization antibody-virus interactions detailed investigations early events infection.