Phage-induced efflux down-regulation boosts antibiotic efficacy

• DOI: 10.1371/journal.ppat.1012361
• Publication Date: 2024-06-28T18:37:22Z
• Authors: Samuel Kraus; Megan L. Fletcher; Urszula Łapińska; Krina Chawla; Evan Baker; Erin L. Attrill; Paul O’Neill; Audrey Farbos; Aaron Jeffries; Edouard E. Galyov; Sunee Korbsrisate; Kay B. Barnes; Sarah V. Harding; Krasimira Tsaneva...; Mark A. T. Blasko...; Stefano Pagliara
• Keywords: Phage therapy; Efflux

The interactions between a virus and its host vary in space time are affected by the presence of molecules that alter physiology either or virus. Determining molecular mechanisms at basis these is paramount for predicting fate bacterial phage populations designing rational phage-antibiotic therapies. We study stationary phase Burkholderia thailandensis ΦBp-AMP1. Although heterogeneous genetic resistance to rapidly emerges B. , enhances efficacy three major antibiotic classes, quinolones, beta-lactams tetracyclines, but antagonizes tetrahydrofolate synthesis inhibitors. discovered enhanced facilitated reduced efflux phage. This new therapy allows eradication bacteria, whilst requiring concentrations, which crucial treating infections sites where it difficult achieve high concentrations.