Cytotoxic Effects of Bulk-Fill Composites on L929 Fibroblast Cells

• DOI: 10.14295/bds.2021.v24i1.2146
• Publication Date: 2021-12-21T22:25:03Z
• Authors: Numan Aydın; Serpil Karaoğlanoğlu; Elif Aybala Oktay; Aysun Kılıç Süloğlu
• Keywords: Viability assay

Objective: Unlike traditional composite resins, bulk-fill resins could be polymerized as thicker layers. This study aims to contribute the field by investigating cytotoxic effects of various on L929 mouse fibroblast cells in vitro. Material and Methods: In our study, six bulk fill one conventional resin were used. Composite samples (8×4 mm) prepared a sterile cabinet using glass mod polymerizing with led light device (DTE LUX E, Germany). (n:3) which surface area was calculated according ISO 10993-12: 2012 standards (3 cm2/ml), kept media for 24 h 72 37 oC incubator, their extracts filtered 1:1 1:2 proportion added cells. Cell viability examined MTT assay cell death LDH test. results evaluated one-way analysis variance (ANOVA) test (p<0.05). Results: When from 4 mm thick applied cells, rates showed significant differences compared control group at end (except Estelite Bulk Fill Flow). Although tested ratio hours caused decrease viability, rate only PRG-containing remained below (70%) specified standards. composites did not produce toxic Beautifil Restorative) Conclusions: Despite decreasing general used layers provided over acceptability level, except (Beautifil Restorative), fibroblasts. Keywords composite; Cytotoxicity; cells; assay.