Distribution and Identification of Pepper yellow leaf curl Indonesia virus Infecting Chili Pepper in Bali Island

• DOI: 10.14692/jfi.17.6.217-224
• Publication Date: 2022-03-25T12:10:33Z
• Authors: Dewa Gede Wiryang...; Suryo Wiyono; Anas Dinurrohman ...; Sri Hendrastuti H...
• Keywords: Leaf curl; Field survey

Yellow leaf curl disease in chili pepper has been reported Bali Island since the early 2012. Research was conducted to identify virus causing this and distribution Bali. Field survey carried out observe intensity collect field samples from several growing areas (Karangasem, Bangli, Tabanan, Gianyar). Begomovirus identification then by polymerase chain reaction method using universal primers SPG1/SPG2, followed an analysis of amplified target DNA sequences. The incidence yellow reached 100% at all sites severity 18%−87%. specific fragment measuring 912 bp successfully 12 samples. Sequence fragments showed highest homology with Pepper Indonesia (PYLCIV). Further phylogenetic confirmed relationship between PYLCIV isolates various Indonesia.