Thiamine leads to oxidative stress resistance via regulation of the glucose metabolism

• DOI: 10.14715/cmb/2019.65.1.13
• Publication Date: 2019-02-20T09:29:32Z
• Authors: Burcu Kartal; Bedia Palabiyik
• Keywords: Transketolase; Pyruvate decarboxylase; Carbohydrate Metabolism

Thiamine diphosphate (ThDP) is an essential cofactor for important enzymes in carbohydrate, amino acid and lipid metabolisms. It also known that thiamine plays role stress response of some organisms. In this study, we focused on the effect responses triggered by various agents. For purpose, firstly, viability Schizosaccharomyces pombe cell cultures was examined under oxidative, osmotic heat stresses. The highest tolerance observed due to presence extracellular (1.5 µM) found only against oxidative stress. Then, enzyme activity catalase superoxide dismutase (SOD) involved antioxidant defense mechanism expression analysis genes encoding related glucose metabolism pathways were investigated condition, it not any difference SOD activities, their gene expressions thiamine, whereas upregulation pyruvate dehydrogenase (pdb1), transketolase (SPBC2G5.05), fructose-1,6-bis-phosphatase (fbp1) downregulation decarboxylase (pdc201) observed. conclusion, these findings suggest leading resistance have impact regulation shifting energy generation from fermentation respiration.