Saline as a vehicle control does not alter ventilation in male CD-1 mice

• DOI: 10.14814/phy2.13702
• Publication Date: 2018-05-30T03:46:36Z
• Authors: Candace N. Receno; Taylor G. Glausen; Lara R. DeRuisseau
• Keywords: Plethysmograph; Hypoxia; Respiratory minute volume; Arterial blood; Hypoxic ventilatory response

Saline (0.9% NaCl) is used in clinical and research settings as a vehicle for intravenous drug administration. While saline standard control mouse studies, there are reports of hyperchloremic metabolic acidosis high doses. It remains unknown if occurs mice and/or compensatory increases breathing frequency tidal volume accompany was hypothesized that administration alters blood pH the pattern conscious CD-1 male exposed to air or hypoxia (10% O2 , balanced N2 ). Unrestrained barometric plethysmography quantify (breaths/min; bpm), (VT; mL/breath/10 g body weight (BW)), minute ventilation (VE; mL/min/10 BW) two designs: (1) 11-week-old with no exposure (n = 11) compared 7 days 0.9% (intraperitoneal, i.p.; 10 mL/kg mass; n 6). (2) 17-week-old tested before (PRE) after 1 day (POST1, 6) (POST7, 5) (i.p.; mass). There were differences when comparing frequency, VT, VE between groups either design room exposures. Hypoxia increased air. Moreover, sampling showed pH, paCO2 paO2 HCO3- without saline. These findings reveal following mice. Therefore, use supported studies evaluating