A NEW approach for characterizing mouse urinary pathophysiologies

• DOI: 10.14814/phy2.14964
• Publication Date: 2021-08-02T07:34:40Z
• Authors: Hannah M. Ruetten; Gervaise H. Henry; Teresa T. Liu; Heidi M. Spratt; William A. Ricke; Douglas W. Strand; Chad M. Vezina
• Keywords: Phenome; Etiology; Hierarchical clustering

The void spot assay (VSA) is a cost-effective method for evaluating and quantifying mouse urinary voiding phenotypes. VSA has been used to differentiate behaviors between experimental groups, but not as diagnostic assay. To build toward this goal, we the define patterns of male mice with diabetic diuresis (BTBR.Cg-Lepob /WiscJ mice), irritative dysfunction (E. coli UTI89 tract infection), obstructive (testosterone estradiol slow-release implants) compared their respective controls. Many studies compare individual endpoints (urine size, quantity, or distribution) groups. Here, consider all collectively establish phenomes three different etiologies dysfunction. We created an approach called normalized endpoint work through (NEW) normalize outputs control mice, then applied principal components analysis hierarchical clustering 12 equally weighted, normalized, scaled, zero-centered outcomes collected from each (the phenome). This accurately classifies based on etiology. show that some aged (>24 m old) develop phenotype while others unique does cluster diabetic, infected, obstructed mice. These findings support use identify specific phenotypes in continued they representative various LUTS men.