Involvement of nucleus accumbens D2–medium spiny neurons projecting to the ventral pallidum in anxiety-like behaviour

• DOI: 10.1503/jpn.220111
• Publication Date: 2023-07-12T12:20:12Z
• Authors: Raquel Correia; Bárbara Coimbra; Ana Verónica Domi...; Marcelina Wezik; Natacha Vieitas-G...; Rita Gaspar; Nuno Sousa; Luísa Pinto; Ana João Rodrigues; Carina Soares-Cunha
• Keywords: Medium spiny neuron; Ventral pallidum

<h3>Background:</h3> The nucleus accumbens (NAcc) is a crucial brain region for emotionally relevant behaviours. NAcc mainly composed of medium spiny neurons (MSNs) expressing either dopamine receptor D1 (D1-MSNs) or D2 (D2-MSNs). D1-MSNs project to the ventral tegmental area (VTA) and pallidum (VP), whereas D2-MSNs only VP. D1- have been associated with depression-like behaviours, but their contribution anxiety remains be determined. <h3>Methods:</h3> We used optogenetic tools selectively manipulate D1-MSN projections from core VP VTA D2-MSN during validated anxiety-producing behavioural procedures in naive mice. In addition, we assessed effects optical stimulation on neuronal activity using vivo electrophysiologic recordings anesthetized animals. <h3>Results:</h3> Optogenetic activation did not trigger anxiety-like behaviour. However, significantly increased This phenotype was decrease putative GABAergic Importantly, pretreating D2-MSN–VP animals γ-aminobutyric acid modulator diazepam prevented optically triggered <h3>Limitations:</h3> exclusive use males tests limits broader interpretation findings. Although conditions that quasi-physiologic changes, there are caveats artificial manipulation activity. <h3>Conclusion:</h3> contributed development behaviour, through modulation