Physically cross-linked scaffold composed of hydroxyapatite-chitosan-alginate-polyamide has potential to trigger bone regeneration in craniofacial defect

• DOI: 10.1515/polyeng-2022-0205
• Publication Date: 2024-02-14T12:30:47Z
• Authors: Md. Masud Rana; Md. Arifuzzaman; Naznin Akhtar; Md. Raziul Haque; Swapan Kumar Sarkar; Md. Nurunnobi; Md. Aliuzzaman Sa...; Sikder M. Asaduzz...
• Keywords: 02 engineering and technology; 0210 nano-technology

Abstract Recently, the fabrication of a scaffold from biomaterials has been increased due to lack adequate natural bone for grafting. In this study, hydroxyapatite-chitosan-alginate-polyamide (HCAP) synthetic was fabricated using thermally induced phase separation (TIPS) technique. The cross-linked with either chemical cross-linker (calcium chloride, 2-hydroxyethyl methacrylate (HEMA), or glutaraldehyde (GTA)) physical (gamma irradiation (IR)) resulting in scaffolds HCAP-CaCl 2 , HCAP-HEMA, HCAP-GTA, and HCAP-IR, respectively. were characterized based on physicochemical properties, cytotoxicity, biocompatibility. HCAP-GTA showed highest density lowest swelling ratio biodegradation rate closely matching those HCAP-IR. Porosity HCAP, 92.14, 87.26, 83.33 %, 0.241, 0.307, 0.335 g/cm 3 same 149, 110, 108 % after 72 h observation. Brine shrimp cytotoxicity RBC biocompatibility assay confirmed non-toxic nature HCAP-IR scaffolds. tested regeneration rabbit mandible defect model. Histological analysis revealed new restoration at site injury. These findings indicate that radiation physically HCAP could be used as an alternative replacement therapy.