Cytotoxic and apoptotic effectiveness of Cypriot honeybee (Apis mellifera cypria) venom on various cancer cells

• DOI: 10.1515/tjb-2023-0109
• Publication Date: 2024-04-10T18:10:32Z
• Authors: Ayşe Nalbantsoy; Ekin Varol; Ayşe Dila Çaglar; Banu Yücel
• Keywords: Bee Venom

Abstract Objectives The bee stinger is the defense organ of honeybees. venom sac a worker connected to its stinger, which used as mechanism, and it has potent complex combination substances that unique in animal kingdom. Many immune-related illnesses have been successfully treated with recent evidence on efficacy applications targeting malignancies attracted considerable attention. Methods 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) test was determine cytotoxicity crude venom, flow cytometric analysis apoptotic potential. cytotoxic activity Apis mellifera cypria collected from two different apiaries Cyprus evaluated for first time against breast (MDA-MB-231), colon (Caco-2), cervix (HeLa), prostate (PC-3), pancreas (Panc-1), lung (A549), glioblastoma (U-87MG) human cancerous healthy fibroblast (CCD-34Lu) cells. Results concentration killed 50 % cells (inhibitory concentration, IC ) expressed cytotoxicity. values A. m. cultured varied 4.18±0.75 22.00±1.71 μg/mL after treatment 48 h, most activities PC-3, Panc-1, HeLa Analysis by cytometry both samples showed slightly induced early apoptosis A549 Panc-1 Conclusions discussed this article, displaying promising results potential source an alternative method because effect.