Seasonal variation in protein content and PLA2 activity of Crotalus molossus molossus venom from captive and wild specimens

• DOI: 10.15174/au.2014.430
• Publication Date: 2015-01-12T15:46:43Z
• Authors: Eduardo Francisco...; Cinthia Osmara Dí...; Ana Bertha Gatica...; Luis Fernando Ple...
• Keywords: Captivity; Crotalus; Ophidia

Envenomation by rattlesnake bites is medical imperative. Medicine focuses on diverse fac­tors such as climate, age, sex, distribution, season of the year and diet, these make snake's venom more poisonous toxic. The objective this study to provide informa­tion seasonal variation protein content hemolytic damage produced captive wild Crotalus molossus molossus. For this, we analyzed 16 samples using colorimetric method visualized Sodium Dodecyl Sulfate-Polyacryl­amide Gel Electrophoresis (SDS-PAGE). activity phospholipase A2 (PLA2) was evaluated erythrocytes-agar plates.We observed a large in con­tent between snake venom; winter pool stands out with highest concentration at 697 μg/μl ± 5.9 μg/μl, followed autumn captivity 648 20.8 spring 528.51 10.59 and, lastly, summer 1 387.9 5.6 μg/μl. PLA2 purified from obtained independent fractions 27, 39, 26, 27 (n=60) spring, summer, winter, respectively. sample showed higher lowest shown sample. We conclude that rattlesnakes concentrations compared snakes, addition there were variations according time year.