Mllt11 Regulates Migration and Neurite Outgrowth of Cortical Projection Neurons during Development

• DOI: 10.1523/jneurosci.0124-22.2022
• Publication Date: 2022-04-04T17:50:16Z
• Authors: Danielle Stanton-...; Karolynn Hsu; Samantha A. Moore; Makiko Yamada; James P. Fawcett; Angelo Iulianella
• Keywords: Neocortex; Neurite; Subplate; Growth cone

The formation of connections within the mammalian neocortex is highly regulated by both extracellular guidance mechanisms and intrinsic gene expression programs. There are two types cortical projection neurons (CPNs): those that project locally interhemispherically to subcerebral structures such as thalamus, hindbrain, spinal cord. regulation morphologies not yet fully understood at molecular level. Here, we report a role for Mllt11 (Myeloid/lymphoid or mixed-lineage leukemia; translocated chromosome 11/All1 Fused Gene From Chromosome 1q) in migration neurite outgrowth callosal during mouse brain formation. We show exclusive developing enriched plate (CP) superficial layers. In cultured primary neurons, detected varicosities growth cones well soma. Using conditional loss-of-function gain-of-function analysis required neuritogenesis proper upper layer CPNs. Loss cortex male female neonates leads severe reduction fibers crossing corpus callosum (CC), progressive loss maintenance neuron expression, reduced complexity dendritic arborization. Proteomic revealed associates with stabilized microtubules, affected microtubule staining axons. Taken together, our findings support promoting mature upper-layer connectivity cerebral cortex. SIGNIFICANCE STATEMENT (CPN) an area active investigation since time Cajal. Yet how complex axonal formed remains incompletely understood. Although mutagenesis mouse, coupled overexpression assays fetal brain, novel protein called sufficient necessary regulate characteristics neocortex. Furthermore, interacts likely accounting its neuritogenesis.