The Canonical Nuclear Factor-κB Pathway Regulates Cell Survival in a Developmental Model of Spinal Cord Motoneurons

• DOI: 10.1523/jneurosci.0206-11.2011
• Publication Date: 2011-04-27T16:49:38Z
• Authors: Stefka Mincheva; Ana Garcera; Myriam Gou-Fabregas; Mario Encinas; Xavier Dolcet; Rosa M. Soler
• Keywords: RELB; IκB kinase

In vivo and in vitro motoneuron survival depends on the support of neurotrophic factors. These factors activate signaling pathways related to cell or inactivate proteins involved neuronal death. present work, we analyzed involvement nuclear factor-κB (NF-κB) pathway mediating mouse spinal cord promoted by This comprises ubiquitously expressed transcription that could be activated two different routes: canonical pathway, associated with IKKα/IKKβ kinase phosphorylation translocation RelA (p65)/p50 factors; noncanonical IKKα homodimer RelB/p52 factor activation. our system, show treatment induced IKKβ translocation, suggesting NF-κB Protein levels members were reduced a primary culture isolated embryonic motoneurons using an interference RNA approach. Even presence factors, selective reduction IKKα, IKKβ, contrast, RelB protein did not have negative effect survival. Together these results demonstrated mediates is this effect. Canonical blockade increase Bim level apoptotic Bcl-x L overexpression Bax counteracted Finally, knockdown causes changes CREB Smn levels.