Aneuploidy and DNA Replication in the Normal Human Brain and Alzheimer's Disease

• DOI: 10.1523/jneurosci.0379-07.2007
• Publication Date: 2007-06-27T17:23:30Z
• Authors: B. Mosch; M. Morawski; A. Mittag; D. Lenz; A. Tarnok; T. Arendt
• Keywords: Cyclin B; Cyclin B1

Reactivation of the cell cycle, including DNA replication, might play a major role in Alzheimer9s disease (AD). A more than diploid content differentiated neurons alternatively result from chromosome mis-segregation during mitosis neuronal progenitor cells. It was our objective to distinguish between these two mechanisms for aneuploidy and provide evidence functional cycle AD. Using slide-based cytometry, chromogenic <i>in situ</i> hybridization, PCR amplification <i>alu</i>-repeats, we quantified amount identified cortical normal human brain AD analyzed link tetraploid expression early mitotic marker cyclin B1. In brain, number with amounts ∼10%. Less 1% contains content. These do not express B1, most likely representing constitutional tetraploidy. This population B1-negative neurons, at reduced number, is also present addition, B1-positive ∼2% all observed Our results indicate that least different need be distinguished giving rise adult brain. Constitutional frequent previously thought. is, however, elevated some have re-entered entirely passed through interphase complete replication.