Synergistic Control of Protein Kinase Cγ Activity by Ionotropic and Metabotropic Glutamate Receptor Inputs in Hippocampal Neurons

• DOI: 10.1523/jneurosci.0478-06.2006
• Publication Date: 2006-03-29T19:42:57Z
• Authors: Franca Codazzi; Alessandra Di Cesare; Nino Chiulli; Alberto Albanese; Tobias Meyer; Daniele Zacchetti; Fabio Grohovaz
• Keywords: Neurons; 0303 health sciences; Neuronal Plasticity; Receptors, Metabotropic Glutamate; Hippocampus; Synaptic Transmission; Rats; Diglycerides; Enzyme Activation; Rats, Sprague-Dawley; 03 medical and health sciences; Receptors, Glutamate; Animals; Calcium; Calcium Signaling; Cells, Cultured; Protein Kinase C

Conventional protein kinase C (PKC) isoforms are abundant neuronal signaling proteins with important roles in regulating synaptic plasticity and other processes. Here, we investigate the role of ionotropic metabotropic glutamate receptor (iGluR mGluR, respectively) activation on generation Ca 2+ diacylglycerol (DAG) signals subsequent neuron-specific PKCγ isoform hippocampal neurons. By combining imaging total internal reflection microscopy analysis specific biosensors, show that elevation both DAG is necessary for sustained translocation EGFP (enhanced green fluorescent protein)-PKCγ. Both production were localized processes, typically observed within discrete microdomains along dendritic branches. Markedly, intermediate-strength NMDA (NMDAR) or moderate electrical stimulation generated but no signals, whereas mGluR signals. receptors needed activation. This suggests a coincidence detection process exists between iGluRs mGluRs relies molecular based corequirement Nevertheless, requirement costimulation could be overcome maximal NMDAR through direct via -sensitive PLCδ (phospholipase Cδ) isoform. In second exception, sufficient neurons which stores loaded by previous activity. Together, dual provides plausible interpretation different synergistic contributions to long-term potentiation