Characterization of Ligands for Fish Taste Receptors

• DOI: 10.1523/jneurosci.0651-07.2007
• Publication Date: 2007-05-23T23:18:51Z
• Authors: Hideaki Oike; Toshitada Nagai; Akira Furuyama; Shinji Okada; Yoshiko Aihara; Yoshiro Ishimaru; Takayuki Marui; Ichiro Matsumoto; Takumi Misaka; Keiko Abe
• Keywords: Oryzias; Taste receptor; Danio; GPR120

Recent progress in the molecular biology of taste reception has revealed that mammals, heteromeric receptors T1R1/3 and T1R2/3 respond to amino acids sweeteners, respectively, whereas T2Rs are for bitter tastants. Similar have also been characterized fish, but their ligands not identified yet. In present study, we conducted a series experiments identify fish receptor ligands. Facial nerve recordings zebrafish (Danio rerio) demonstrated perceived even denatonium, which is representative aversive compounds mammals Drosophila. Calcium imaging analysis T1Rs medaka (Oryzias latipes) using an HEK293T heterologous expression system both responded sugars. A triple-labeling, situ hybridization cells expressing T1R2/3s exist PLCbeta2-expressing bud fish. Functional showed zfT2R5 mfT2R1 denatonium. Behavior observations confirmed prefer avoid These results suggest that, although there may be some fish-specific way discriminating ligands, vertebrates could conserved gustatory mechanism by attractive tastants, respectively.