Dual intrinsic and extrinsic origins of CGRP- and NPY-immunoreactive nerves of rat gut and pancreas

• DOI: 10.1523/jneurosci.07-09-02674.1987
• Publication Date: 2018-03-30T21:00:33Z
• Authors: HC Su; AE Bishop; RF Power; Y Hamada; JM Polak
• Keywords: 0301 basic medicine; Histocytochemistry; Calcitonin Gene-Related Peptide; Neuropeptides; Stomach; Galanin; Rats, Inbred Strains; Substance P; Denervation; Rats; 03 medical and health sciences; Ganglia, Spinal; Animals; Neuropeptide Y; Peptides; Pancreas; Vasoactive Intestinal Peptide

he origins of nerves containing calcitonin gene-related peptide (CGRP), neuropeptide Y (NPY), and substance P were investigated in the rat stomach, pancreas, colon, using immunocytochemistry combined with retrograde tracing surgical chemical denervation procedures. Compared vasoactive intestinal polypeptide (VIP) galanin, which have primarily local mammalian gut, CGRP-, NPY-, P-immunoreactive revealed dual extrinsic intrinsic origins. Immunocytochemistry showed that CGRP- stomach pancreas originate from bilateral dorsal root ganglia mainly at levels T8-T11, while those colon are derived S1 and, to a lesser extent, L1 L6. Chemical denervations neurons these form sensory input CGRP largest proportion. The results indicated NPY-immunoreactive postganglionic sympathetic lying coeliac inferior mesenteric ganglia. These located around blood vessels gut sensitivity 6-hydroxydopamine, thus likely be noradrenergic. present study provides detailed mapping some major peptide-containing gastroenteropancreatic tract, providing further information on anatomy enteric innervation.