Multiple nicotinic acetylcholine receptor genes are expressed in goldfish retina and tectum

• DOI: 10.1523/jneurosci.10-02-00670.1990
• Publication Date: 2018-04-02T21:55:42Z
• Authors: K Cauley; BW Agranoff; D Goldman
• Keywords: Tectum; Ganglion cell layer; Alpha (finance)

cDNAs encoding a novel nAChR structural subunit (GFn alpha-3) and ligand-binding (GF have been isolated from goldfish retina cDNA library. The protein encoded by GFn alpha-3 shares 88% amino acid similarity with that alpha-2, gene previously identified to be expressed in this system (Cauley et al., 1989). is likely the homolog of rat (Boulter 1986). Northern blots S1 protection experiments show GF genes are brain. identifies multiple RNAs differing their 3' untranslated regions. In situ hybridization analysis demonstrates alpha-3, expression cells retinal ganglion cell layer. Unlike alpha-2 at highest levels retina's inner nuclear optic tectum, both periventricular zone, as well more superficial layers. These results suggest presence systems tectum. addition, they indicate tectal nAChRs may arise remote (ganglion cell) local (tectal synthesis.