EBF1 Limits the Numbers of Cochlear Hair and Supporting Cells and Forms the Scala Tympani and Spiral Limbus during Inner Ear Development

• DOI: 10.1523/jneurosci.1060-23.2023
• Publication Date: 2024-01-10T05:32:04Z
• Authors: Hiroki Kagoshima; Hiroe Ohnishi; Ryosuke Yamamoto; Akiyoshi Yasumoto; Yosuke Tona; Takayuki Nakagawa; Koichi Omori; Norio Yamamoto
• Keywords: Cochlear duct; Scala

Early B-cell factor 1 (EBF1) is a basic helix-loop-helix transcription essential for the differentiation of various tissues. Our single-cell RNA sequencing data suggest that Ebf1 expressed in sensory epithelium mouse inner ear. Here, we found murine gene and its protein are prosensory domain ear, medial region cochlear duct floor, otic mesenchyme, cochleo-vestibular ganglion. deletion mice results incomplete formation spiral limbus scala tympani, increased number cells organ Corti Kölliker's organ, aberrant course ganglion axons. epithelia caused proliferation SOX2-positive at E13.5, indicating EBF1 suppresses to facilitate development appropriate numbers hair supporting cells. Furthermore, with epithelium-specific showed poor postnatal hearing function. normal auditory function mammals. Significance statement The elaborate cellular organization three-layered luminal structure mammalian cochlea sound perception, but developmental process these structures not fully understood. present study revealed roles type cochlea. was widely regulated proper cells, involved developing tympani limbus. As result, necessary hearing. These whole contribute understanding part complex process.