G(olf) and Gs in rat basal ganglia: possible involvement of G(olf) in the coupling of dopamine D1 receptor with adenylyl cyclase

• DOI: 10.1523/jneurosci.13-05-02237.1993
• Publication Date: 2018-03-31T01:00:27Z
• Authors: D Herve; M Levi-Strauss; I Marey-Semper; C Verney; JP Tassin; J Glowinski; JA Girault
• Keywords: Male; Neurons; 0303 health sciences; Dopamine; Receptors, Dopamine D1; Brain; Quinolinic Acid; Immunohistochemistry; Basal Ganglia; Corpus Striatum; Rats; Rats, Sprague-Dawley; Ribosome Inactivating Proteins, Type 2; 03 medical and health sciences; GTP-Binding Proteins; Animals; RNA, Messenger; Plant Lectins; N-Glycosyl Hydrolases; Adenylyl Cyclases; Glycoproteins; Plant Proteins

Using specific antibodies and cDNA probes, we have investigated, in rat basal ganglia, the distribution regulation of expression alpha subunits Gs G(olf), two GTP-binding proteins (G-proteins) that stimulate adenylyl cyclase. We confirmed G(olf) is highly expressed caudate-putamen, nucleus accumbens, olfactory tubercle, whereas less abundant these areas than other brain regions. Intrastriatal injections quinolinic acid decreased dramatically levels protein striatum substantia nigra, those mRNA striatum. Retrograde lesions striatonigral neurons with volkensin reduced markedly D1 dopamine (DA) binding sites, as well striatum, without altering D2 sites. In contrast, both types increased nigra. Immunocytochemistry showed presence striatal medium-sized several neuronal populations. These results demonstrate contain high little, if any, alpha, suggesting coupling receptor to cyclase provided by alpha. The were five- sixfold higher indicating a preferential localization somatodendritic region providing basis for low efficiency Six weeks after 6-hydroxydopamine DA neurons, an increase (+53%) (+64%) was observed This may account DA-activated supersensitivity, change receptors density, follows destruction neurons. Fine physiological or pathological situations be critical parameter neurotransmission.