Modulation of gap junctional mechanisms during calcium-free induced field burst activity: a possible role for electrotonic coupling in epileptogenesis

• DOI: 10.1523/jneurosci.14-07-04308.1994
• Publication Date: 2018-04-02T17:50:35Z
• Authors: JL Perez-Velazquez; TA Valiante; PL Carlen
• Keywords: Bursting; Lucifer yellow; Carbenoxolone; Premovement neuronal activity

To date, there is little experimental evidence supporting or refuting electrotonic interactions through gap junctions in the generation and/or spread of seizure activity mammalian brain. We have studied junctional mechanisms vitro calcium-free induced model epilepsy using electrophysiological and staining techniques CA1 area hippocampus. Lucifer yellow pyramidal neurons revealed that dye coupling was increased 2.3 times hippocampal slices made hyperexcitable by perfusion with artificial cerebrospinal fluid (aCSF). Furthermore, multiple neuronal (triplets, quintuplets) observed these conditions but never control (standard aCSF). Under reduce conductance (intracellular acidification, octanol, halothane), seizure-like suppressed this model, whereas increasing intracellular alkalinization frequency duration field burst events. Intracellular acidification also reduced as well fast prepotentials (electrotonic potentials) without altering firing frequency. Simultaneous extracellular single whole-cell recordings suppression synchronization between spontaneous during acidification. These observations indicate an apparent increase rhythmic hippocampus may contribute substantially to underlying