Protein synthesis-dependent formation of protein kinase Mzeta in long- term potentiation

• DOI: 10.1523/jneurosci.16-08-02444.1996
• Publication Date: 2018-04-05T22:28:20Z
• Authors: P Osten; L Valsamis; A Harris; TC Sacktor
• Keywords: Anisomycin; Tetanic stimulation

The maintenance of long-term potentiation (LTP) in the CA1 region hippocampus has been reported to require both a persistent increase phosphorylation and synthesis new proteins. increased activity protein kinase C (PKC) during phase LTP may result from formation PKMzeta, constitutively active fragment specific PKC isozyme. To define relationship among EPSP responses, requirement for synthesis, we examined regulation PKMzeta after sub-threshold stimulation that produced short-term (STP) suprathreshold by single multiple tetanic trains LTP. We found that, although no followed STP, degree was linearly correlated with PKMzeta. first observed 10 min tetanus induced lasted at least 2 hr, parallel persistence enhancement. Both PKMzeta++ were blocked inhibitors anisomycin cycloheximide. These results suggest is component synthesis-dependent mechanism