Estimate of the Chloride Concentration in a Central Glutamatergic Terminal: A Gramicidin Perforated-Patch Study on the Calyx of Held

• DOI: 10.1523/jneurosci.1660-06.2006
• Publication Date: 2006-11-02T18:08:27Z
• Authors: Gareth D. Price; Laurence O. Trussell
• Keywords: Mice, Inbred ICR; 0303 health sciences; Patch-Clamp Techniques; Glycine; Gramicidin; Presynaptic Terminals; Action Potentials; Glutamic Acid; In Vitro Techniques; Rats; Mice; 03 medical and health sciences; Chlorides; Animals; Rats, Wistar; Brain Stem

The function of presynaptic terminals is regulated by intracellular Cl − , the levels which modify vesicular endocytosis and transmitter refilling mediate effects ligand-gated channels. Nevertheless, concentration in a central nerve terminal unknown, it unclear whether can regulate independently soma. Using perforated-patch recording mammalian synapse, we found that accumulate up to 21 m between four five times higher than their parent cell bodies. Changing [Cl ] did not alter glutamate content intact terminals, unlike vitro experiments. Thus, glutamatergic maintain an elevated without compromising synaptic transmission.