The Mammalian Staufen Protein Localizes to the Somatodendritic Domain of Cultured Hippocampal Neurons: Implications for Its Involvement in mRNA Transport

• DOI: 10.1523/jneurosci.19-01-00288.1999
• Publication Date: 2018-04-06T20:50:42Z
• Authors: Michael A. Kiebler; Indradeo Hemraj; Paul Verkade; Martin Köhrmann; Puri Fortes; Rosa M. Marión; Juan Ortı́n; Carlos G. Dotti
• Keywords: Colocalization; Immunoelectron microscopy

In hippocampal neurons, certain mRNAs have been found in dendrites (), and their localization translation implicated synaptic plasticity (). One attractive candidate to achieve transport of into is Staufen (Stau), a double-stranded RNA-binding protein, which plays pivotal role mRNA transport, localization, Drosophila Using antibodies raised against peptide located the domain IIa polyclonal antibody recently cloned human homolog, we identify 65 kDa rat homolog cultured neurons. agreement with exclusive somatodendritic these cells, find that restricted same domain. By immunoelectron microscopy, show enrichment mammalian Stau (mStau) vicinity smooth endoplasmic reticulum microtubules near contacts. Finally, association mStau neuronal suggested by colocalization ribonucleoprotein particles specifically distal known contain mRNA, ribosomes, factors These results suggest for polarized