A Role for HSP27 in Sensory Neuron Survival

• DOI: 10.1523/jneurosci.19-20-08945.1999
• Publication Date: 2018-04-05T00:55:53Z
• Authors: Susan E. Lewis; Richard J. Mannion; Fletcher A. White; Richard E. Cogges...; Simon Beggs; Michael Costigan; Jody L. Martin; Wolfgang H. Dillmann; Clifford J. Woolf
• Keywords: Axotomy; Hsp27; Sensory neuron; Peripheral nerve injury

Peripheral nerve injury in neonatal rats results the death of majority axotomized sensory neurons by 7 d after injury. In adult animals, however, all survive for at least 4 months axotomy. How acquire capacity to axonal is not known. Here we describe how expression small heat shock protein 27 (HSP27) correlated with neuronal survival axotomy vivo and NGF withdrawal vitro. The number HSP27-immunoreactive L4 DRG low birth does change significantly 21 postnatal day 0 (P0) sciatic contrast, begin express HSP27. One week P0 section total dramatically reduced, but surviving neurons, as identified c-jun immunoreactivity, are immunoreactive addition, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling reveals that very few HSP27-expressing dying 48 hr vitro , a similar correlation exists between HSP27 survival; cultures, preferentially withdrawal. Finally, overexpression human rat sympathetic increases withdrawal, nearly twice many hr. Together these suggest plays role promoting or neurotrophin