Brain-Derived Neurotrophic Factor Is Released in the Dorsal Horn by Distinctive Patterns of Afferent Fiber Stimulation

• DOI: 10.1523/jneurosci.21-12-04469.2001
• Publication Date: 2018-04-05T02:15:54Z
• Authors: Isobel J. Lever; Elizabeth J. Brad...; Joanna R. Cunningham; David W. Adelson; Martyn G. Jones; Stephen B. McMahon; Juan Carlos G. Ma...; Marzia Malcangio
• Keywords: Capsaicin; Tetanic stimulation

Brain-derived neurotrophic factor (BDNF) is synthesized by small neuron cell bodies in the dorsal root ganglia (DRG) and anterogradely transported to primary afferent terminals horn where it involved modulation of painful stimuli. Here we show that BDNF released rat isolated after chemical stimulation capsaicin or electrical roots. Capsaicin superfusion (1–100 μ m ) induced a dose-dependent release BDNF, measured using ELISA. The highest dose also depletion protein horn. was seen roots at C-fiber strength. This encoded specific patterns fiber stimulation. Neither continuous low-frequency (480 pulses, 1 Hz) nor tetanic high-frequency (300 pulses 3 trains, 100 evoked although substance P (SP) observed under both these conditions. However, short bursts 75 along with SP glutamate. NMDA antagonist d -AP-5 inhibited electrically release. systemic intrathecal NGF treatment. upregulated content DRG increased capsaicin-evoked BDNF. Similarly, amount burst activity-dependent continued be solely this pattern. These experiments demonstrate mediated receptor activation.