A High-Efficiency Protein Transduction System Demonstrating the Role of PKA in Long-Lasting Long-Term Potentiation

• DOI: 10.1523/jneurosci.21-16-06000.2001
• Publication Date: 2018-04-06T19:30:20Z
• Authors: Masayuki Matsushita; Kazuhito Tomizawa; Akiyoshi Moriwaki; Sheng-Tian Li; Hiroaki Terada; Hideki Matsui
• Keywords: Transduction (biophysics); Compartment (ship)

Proteins and peptides have been demonstrated to penetrate across the plasma membrane of eukaryotic cells by protein transduction domains. We show that 11 arginine (<i>11R</i>) is an efficient method delivering proteins into neurons brain slices. Here, we demonstrate PKA inhibitory peptide, fused with <i>11R</i> nuclear localization signal, delivers peptide exclusively compartment in This blocked both cAMP responsive element-binding phosphorylation long-lasting long-term potentiation (LTP) induction, but not early LTP. These results highlight specific neuronal subcellular compartments slices as a powerful tool for studying plasticity.