Light Stimulates a Transducin-Independent Increase of Cytoplasmic Ca2+and Suppression of Current in Cones from the Zebrafish Mutantnof

• DOI: 10.1523/jneurosci.23-02-00470.2003
• Publication Date: 2018-04-06T20:00:22Z
• Authors: Susan E. Brockerhoff; Fred Rieke; Hugh R. Matthews; Michael R. Taylor; Breandan Kennedy; Irina Ankoudinova; Gregory A. Niemi; Chandra L. Tucker; Ming Xiao; Marianne C. Cilluffo; Gordon L. Fain; James B. Hurley
• Keywords: Transducin; Visual phototransduction

Transducins couple visual pigments to cGMP hydrolysis, the only recognized phototransduction pathway in vertebrate photoreceptors. Here we describe a zebrafish mutant, no optokinetic response f(w21) (nof), with nonsense mutation gene encoding alpha subunit of cone transducin. Retinal morphology and levels enzymes are normal nof retinas, but transducin is undetectable. Dark current cones also normal, it insensitive moderate intensity light. The do respond, however, bright These responses produced by light-stimulated, transducin-independent, release Ca2+ into cytoplasm. Thus, addition stimulating transducin, light independently induces photoreceptor