Intracranially Administered Anti-Αβ Antibodies Reduce β-Amyloid Deposition by Mechanisms Both Independent of and Associated with Microglial Activation

• DOI: 10.1523/jneurosci.23-09-03745.2003
• Publication Date: 2018-04-13T22:10:41Z
• Authors: Donna M. Wilcock; Giovanni DiCarlo; Debbi Henderson; Jennifer Jackson; Keisha Clarke; Kenneth E. Ugen; Marcia N. Gordon; Dave Morgan
• Keywords: Immunostaining; Amyloid (mycology)

Active immunization against the β-amyloid peptide (Αβ) with vaccines or passive systemic monoclonal anti-Aβ antibodies reduces amyloid deposition and improves cognition in APP transgenic mice. In this report, intracranial administration of anti-Αβ into frontal cortex hippocampus Tg2576 mice is described. The antibody injection resulted initially a broad distribution staining for antibody, which diminished over 7 d. Although no loss immunostaining deposited Aβ was apparent at 4 hr, dramatic reduction Αβ load discernible 24 hr maintained 3 A thioflavine-S-positive compact plaque delayed until d, time microglial activation also became apparent. At 1 week after injection, returned to control levels, whereas thioflavine-S remained reduced. results from study suggest two-phase mechanism action. first phase occurs between clears primarily diffuse deposits, not associated observable activation. second responsible clearance are discussed context other studies identifying coincident removal animals.