Key Electrophysiological, Molecular, and Metabolic Signatures of Sleep and Wakefulness Revealed in Primary Cortical Cultures

Sleep
DOI: 10.1523/jneurosci.2306-12.2012 Publication Date: 2012-09-05T16:38:31Z
ABSTRACT
Although sleep is defined as a behavioral state, at the cortical level has local and use-dependent features suggesting that it property of neuronal assemblies requiring in function activation experienced during prior wakefulness. Here we show mature cultured neurons display default state characterized by synchronized burst-pause firing activity reminiscent sleep. This sleep-like can be changed to transient tonic wakefulness when cultures are stimulated with mixture waking neurotransmitters spontaneously returns state. In addition electrophysiological similarities, transcriptome strikingly resembles sleep-deprived mice, plastic changes reflected AMPA receptors phosphorylation also similar. We used our vitro model animals map metabolic pathways activated waking. Only few were identified, including glycolysis, aminoacid, lipids. Unexpectedly large increases lysolipids found both vivo after deprivation stimulation, strongly might play major role reestablishing membrane homeostasis. With model, cellular molecular consequences now investigated dish.
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